Integrative diagnosis and molecular phylogeny of dagger and needle nematodes of olives and grapevines in the island of Crete, Greece, with description of Xiphinema cretense n. sp. (Nematoda, Longidoridae)

  title={Integrative diagnosis and molecular phylogeny of dagger and needle nematodes of olives and grapevines in the island of Crete, Greece, with description of Xiphinema cretense n. sp. (Nematoda, Longidoridae)},
  author={Emmanuel A. Tzortzakakis and Antonio Archidona‐Yuste and Carolina Cantalapiedra‐Navarrete and Eleni Nasiou and Maria S. Lazanaki and Emmanouil M. Kabourakis and Juan Emilio Palomares-Rius and Pablo Castillo},
  journal={European Journal of Plant Pathology},
The occurrence and geographic distribution of longidorid nematode species inhabiting the rhizosphere of cultivated and wild olive and grapevine in Crete Island were investigated. Morphological and morphometrical studies identified five Longidorus and six Xiphinema species, with frequencies of prevalence (for wild and cultivated olives and grapevines, respectively) as follows: Longidorus closelongatus (2.0–13.3 %), L. cretensis (1.0–6.7 %), L. moesicus (13.3 % only in grapevines), L. orientalis… 
Description of the first-stage juveniles of Xiphinema cretense and X. herakliense - Distribution of Xiphinema and Longidorus species in olive orchards and grapevines in Crete, Greece
Summary The occurrence of nematodes of the family Longidoridae was investigated in soil samples collected from cultivated and wild olives and grapevines in Crete. The first-stage juveniles of
Remarkable Diversity and Prevalence of Dagger Nematodes of the Genus Xiphinema Cobb, 1913 (Nematoda: Longidoridae) in Olives Revealed by Integrative Approaches
This study makes available the first detailed information on the diversity and distribution of Xiphinema species infesting wild and cultivated olive in a wide-region in southern Spain and develops a comparative morphological and morphometrical study together with molecular data from three nuclear ribosomal RNA genes.
Molecular phylogenetic analysis and comparative morphology resolve two new species of olive-tree soil related dagger nematodes of the genus Xiphinema (Dorylaimida : Longidoridae) from Spain
This study describes two new species of Xiphinema from the Iberian Peninsula associated with cultivated and wild olive trees and describes a well developed pseudo-Z-organ, characterised by an odontostyle and odontophore 136–149 and 65–80 µm long, respectively, and aWell developed Z-organ with refractive inclusions, variable in shape.
Rotylenchus cretensis n. sp. and R. cypriensis Antoniou 1980 (Nematoda: Hoplolaimidae) recovered from the rhizosphere of olive at Crete (Greece) with a molecular phylogeny of the genus
The results of phylogenetic analyses based on D2-D3 region, ITS of rRNA, and the partial 18S sequences confirmed the close molecular relationships between R. urmiaensis and R. striaticeps, and clearly support the proposal of R. cretensis n.
Description of Xiphinema azarbaijanense n. sp. (Nematoda; Longidoridae) from West Azarbaijan province, northwestern Iran
A new species of the dagger nematode genus Xiphinema was recovered from three different points in West Azarbaijan province, northwestern Iran, from the rhizosphere of grasses and described based on
Genetic diversity, and description of a new dagger nematode, Xiphinema afratakhtehnsis sp. nov., (Dorylaimida: Longidoridae) in natural forests of southeastern Gorgan, northern Iran
The most prevalent dagger nematode recovered from rhizospheric soil samples of forest trees in the Afrātakhteh region of Golestan province (Iran) was Xiphinema afratakhtehnsis sp. nov. and it is
Xiphinema japonicum n. sp. (Nematoda: Longidorinae) from the Rhizosphere of Japanese Podocarpus macrophyllus (Thunb.), a Cryptic Species Related to Xiphinema bakeri Williams, 1961
Abstract Xiphinema japonicum n. sp., isolated in Ningbo, China, from the rhizosphere of Podocarpus macrophyllus (Thunb.) imported from Japan is described. The new species belongs to Xiphinema
Unravelling the Biodiversity and Molecular Phylogeny of Needle Nematodes of the Genus Longidorus (Nematoda: Longidoridae) in Olive and a Description of Six New Species
This study provides the first detailed information on the diversity and distribution of Longidorus species infesting wild and cultivated olive soils in a wide-region in southern Spain that included 159 locations from which 449 sampling sites were analyzed and represents the most complete phylogenetic analysis for Longidorous species to date.
First record and description of juvenile stages of Longidorus artemisiae Rubtsova, Chizhov & Subbotin, 1999 (Nematoda: Longidoridae) in Poland and new data on L. juglandicola Lišková, Robbins & Brown, 1997 based on topotype specimens from Slovakia
This paper presents the first geographical record of the needle nematode Longidorus artemisiae Rubtsova, Chizhov & Subbotin, 1999 outside Russia, found in Poland near the city of Skierniewice in association with nettle, including the first description of juveniles of this species.
Redescription and molecular characterisation of Xiphinema barense Lamberti et al., 1986 (Nematoda: Longidoridae) from wild olive trees in southern Italy
The phylogenetic analyses based on the sequences of the D2-D3 expansion regions of the 28S rRNA gene and ITS rRNA genes confirm the species differentiation and indicate the phylogenetic position of X. barense and its relationship with closely related species.


Prevalence, polyphasic identification, and molecular phylogeny of dagger and needle nematodes infesting vineyards in southern Spain
Nematode surveys were conducted on 77 vineyards during the spring seasons of 2006, 2007 and 2008 in the main Andalusian grapevine-growing areas, including the provinces of Cádiz, Córdoba, and Huelva to reconstruct phylogenetic relationships among these species and with other longidorids.
Longidorus cretensis n. sp. (Nematoda: Longidoridae) from a vineyard infected with a foliar `yellow mosaic' on Crete, Greece
A description is provided of Longidorus cretensis n. sp., a bisexual species associated with grapevines in Crete, Greece. The species is characterised by a body length of 6.3-8.7 mm, head flattened
A New Root-Knot Nematode, Meloidogyne baetica n. sp. (Nematoda: Heteroderidae), Parasitizing Wild Olive in Southern Spain.
Phylogenetic trees derived from maximum parsimony analyses showed that M. baetica is closely related to M. artiellia, the cereal and legume root-knot nematode, and the ability of this new nematodes to infect wild and cultivated olives only is suggested.
Multiplex Polymerase Chain Reaction Identification of Single Individuals of the Longidorid Nematodes Xiphinema index, X. diversicaudatum, X. vuittenezi, and X. italiae Using Specific Primers from Ribosomal Genes.
The sequence variability made it possible to design species-specific internal sense primers that amplified, in combination with the same antisense ITS1 primer, a single signature fragment, which will be particularly useful for successful nematode management practices in vineyards.
Description and molecular characterisation of Paralongidorus litoralis sp. n. and P. paramaximus Heyns, 1965 (Nematoda: Longidoridae) from Spain
Paralongidorus litoralis sp. n., a new bisexual species of the genus, is described and illustrated by light microscopy, scanning electron microscopy and molecular studies from specimens collected in
Nematode vectors of plant viruses in New Zealand
Abstract A survey of virus vector nematodes based on a total of 320 published and recent records revealed that 3 Longidorus, 1 Paralongidorus, 6 Xiphinema, 2 Trichodoms, and 3 Paratrichodorus species
Longidorus israelensis sp.n. (Nematoda: Dorylaimoidea), a parasite of carrot in Israel.
A parthenogentic species associated with diseased carrots in Israel, Longidorus israelensis sp.n, is described, characterised by its long body, slightly expanded and anteriorly flattened head region, amphidialpouches not bilobed, long odontostyle, and almost hemispherical short tail.
Longidorids (Nematoda: Dorylaimoidea) occurring in the rhizosphere of olive trees in Western Crete, Greece.
Of 50 soil samples collected from the rhizosphere of olive, grapevine, carob or walnut in the Province of Chania, Crete, 42 were positive for Longidoridae, and the four juvenile stages of this species are clearly separated on the basis of their odontostyle and replacement odontOSTyle length.
The Who , What and Where of Longidoridae and Trichodoridae
The main diagnostic features of the Longidoridae at the genus level are the structure of the odontostyle and odontophore, the structure and position of the guide ring, the shape of the amphidial fovea, shape and size of amphid opening and the position and sizes of the pharyngeal gland nuclei.
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