Integration site analysis in transgenic mice by thermal asymmetric interlaced (TAIL)-PCR: segregating multiple-integrant founder lines and determining zygosity

@article{Pillai2007IntegrationSA,
  title={Integration site analysis in transgenic mice by thermal asymmetric interlaced (TAIL)-PCR: segregating multiple-integrant founder lines and determining zygosity},
  author={Manoj M Pillai and Gopalakrishnan M. Venkataraman and Steven T. Kosak and Beverly J Torok-Storb},
  journal={Transgenic Research},
  year={2007},
  volume={17},
  pages={749-754}
}
When transgenic mice are created by microinjection of DNA into the pronucleus, the sites of DNA integration into the mouse genome cannot be predicted. Most methods based on polymerase chain reaction (PCR) that have been used for determining the integration site of foreign DNA into a genome require specific reagents and/or complicated manipulations making routine use tedious. In this report we demonstrate the use of a PCR-based method—TAIL-PCR (Thermal Asymmetric Interlaced PCR) which relies on… CONTINUE READING

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