Integration of the information from gene expression and metabolic fluxes for the analysis of the regulatory mechanisms in Synechocystis

@article{Yang2002IntegrationOT,
  title={Integration of the information from gene expression and metabolic fluxes for the analysis of the regulatory mechanisms in Synechocystis},
  author={Ching-hong Yang and Qiang Hua and Kazuyuki Shimizu},
  journal={Applied Microbiology and Biotechnology},
  year={2002},
  volume={58},
  pages={813-822}
}
Synechocystis was grown under autotrophic, mixotrophic and heterotrophic conditions, and the gene expression patterns at the mRNA and protein levels were measured by using semi-quantitative RT-PCR and two-dimensional electrophoresis (2DE), respectively. Moreover, the intracellular metabolic flux distributions in Synechocystis grown under different trophic conditions were also determined using the carbon isotope labeling technique. By combining the information obtained from the transcript levels… CONTINUE READING

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