Integration of tetracycline regulation into a cell-specific transcriptional enhancer.

@article{Rose1997IntegrationOT,
  title={Integration of tetracycline regulation into a cell-specific transcriptional enhancer.},
  author={Stephen D Rose and Raymond J MacDonald},
  journal={The Journal of biological chemistry},
  year={1997},
  volume={272 8},
  pages={4735-9}
}
The pancreas-specific transcriptional enhancer of the rat elastase I gene was modified by substituting, in turn, each of its three individual constitutive elements with the tetO element, which confers regulation by exogenous tetracycline in the presence of the hybrid tetO binding transactivator (tTA). Whereas the unmodified enhancer was active in transfected acinar tumor cells, substitution of individual elements with the tet-responsive element abolished activity. The modified enhancers were… CONTINUE READING