Integration of growth factor gene delivery with collagen‐triggered wound repair cascades using collagen‐mimetic peptides

@article{Urello2016IntegrationOG,
  title={Integration of growth factor gene delivery with collagen‐triggered wound repair cascades using collagen‐mimetic peptides},
  author={Morgan A. Urello and Kristi L. Kiick and Millicent O. Sullivan},
  journal={Bioengineering \& Translational Medicine},
  year={2016},
  volume={1},
  pages={207 - 219}
}
Abstract Growth factors (GFs) play vital roles in wound repair. Many GF therapies have reached clinical trials, but success has been hindered by safety concerns and a lack of efficacy. Previously, we presented an approach to produce protein factors in wound beds through localized gene delivery mediated by biomimetic peptides. Modification of polyethylenimine (PEI) DNA polyplexes with collagen‐mimetic peptides (CMPs) enabled tailoring of polyplex release/retention and improved gene transfer… 

Figures from this paper

Enhanced wound healing via collagen-turnover-driven transfer of PDGF-BB gene in a murine wound model.
TLDR
The combined properties of the co-gels, including their stability and capacity to control both cell recruitment and cell phenotype within the murine wound bed, strongly supports the potential ofThe co-gel scaffolds for improved treatment of chronic non-healing wounds.
Biomaterial substrate modifications that influence cell-material interactions to prime cellular responses to nonviral gene delivery
TLDR
How biomaterial substrate modifications can prime the cellular response to nonviral gene delivery (e.g. integrin binding and focal adhesion formation, cytoskeletal remodeling, endocytic mechanisms, and intracellular trafficking) is summarized to aid in improving gene delivery for future therapeutic applications.
Therapeutic nanocarriers comprising extracellular matrix-inspired peptides and polysaccharides
TLDR
This review highlights recent developments of ECM-inspired nanocarriers and their exploration for drug and gene delivery applications and includes an update on current clinical trials employing elastin-like proteins, a well-established tolerability and efficacy track record in preclinical analyses.
Targeted Drug Delivery via the Use of ECM-Mimetic Materials
TLDR
An overview of cellular receptors that interact with ECM molecules is provided and the potential impacts of utilizing the interaction between ECM components and cellular receptors for intracellular delivery, particularly in tissue regeneration applications are highlighted.
Gene delivery by peptide-assisted transport.
Bio-Hybrid Hydrogels Incorporated into a System of Salicylic Acid-pH/Thermosensitive Nanocarriers Intended for Cutaneous Wound-Healing Processes
TLDR
The obtained results indicated that the pH/thermosensitive nanocarrier–salicylic acid system and bio-hybrid hydrogels are characterized by antibacterial activity against both S. aureus and E. coli.
Biopolymeric systems for the delivery of nucleic acids
Hybrid hydrogels for biomedical applications.
...
1
2
...

References

SHOWING 1-10 OF 73 REFERENCES
Matrix immobilization enhances the tissue repair activity of growth factor gene therapy vectors.
TLDR
It is demonstrated that immobilizing matrices enable the controlled delivery and activity of tissue promoting genes for the effective regeneration of injured tissues.
Adenovirus encoding human platelet-derived growth factor-B delivered in collagen exhibits safety, biodistribution, and immunogenicity profiles favorable for clinical use.
  • D. Gu, T. Nguyen, L. Chandler
  • Biology, Medicine
    Molecular therapy : the journal of the American Society of Gene Therapy
  • 2004
Collagen-embedded platelet-derived growth factor DNA plasmid promotes wound healing in a dermal ulcer model.
TLDR
PDGF DNA gene therapy is effective at accelerating wound healing in ischemic dermal ulcers and provides a viable alternative to peptide growth factor therapy.
Dual growth factor releasing multi-functional nanofibers for wound healing.
Non-viral gene-activated matrices
TLDR
Having successfully developed gene-activated matrices using non-viral gene therapy in combination with collagen-based scaffolds engineered specifically for tissue repair, the ephrinB2 gene has recently been incorporated to produce a novel therapeutic GAM for bone repair.
Particle-mediated gene transfer of PDGF isoforms promotes wound repair.
TLDR
It is demonstrated that in vivo gene transfer by particle bombardment can be used to improve the tissue repair response and will aid in the development of therapeutic cutaneous gene delivery.
Adenoviral mediated gene transfer of PDGF‐B enhances wound healing in type I and type II diabetic wounds
  • S. Keswani, A. B. Katz, T. Crombleholme
  • Medicine, Biology
    Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society
  • 2004
TLDR
Adenoviral‐PDGF‐B treatment significantly enhanced epithelial gap closure in db/db, streptozotocin, and nonobese NOD/Ltj mice as compared to diabetic phosphate buffered saline solution or Ad LacZ controls.
...
1
2
3
4
5
...