Integration of flux measurements to resolve changes in anabolic and catabolic metabolism in cardiac myocytes.

@article{Gibb2017IntegrationOF,
  title={Integration of flux measurements to resolve changes in anabolic and catabolic metabolism in cardiac myocytes.},
  author={Andrew Alan Gibb and Pawel K. Lorkiewicz and Yu-Ting Zheng and Xiang Zhang and Aruni Bhatnagar and Steven P Jones and Bradford G. Hill},
  journal={The Biochemical journal},
  year={2017},
  volume={474 16},
  pages={
          2785-2801
        }
}
Although ancillary pathways of glucose metabolism are critical for synthesizing cellular building blocks and modulating stress responses, how they are regulated remains unclear. In the present study, we used radiometric glycolysis assays, [13C6]-glucose isotope tracing, and extracellular flux analysis to understand how phosphofructokinase (PFK)-mediated changes in glycolysis regulate glucose carbon partitioning into catabolic and anabolic pathways. Expression of kinase-deficient or phosphatase… CONTINUE READING
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