Integration of Agrobacterium tumefaciens transfer DNA (T-DNA) involves rearrangements of target plant DNA sequences.

@article{Gheysen1987IntegrationOA,
  title={Integration of Agrobacterium tumefaciens transfer DNA (T-DNA) involves rearrangements of target plant DNA sequences.},
  author={Godelieve Gheysen and Marc Van Montagu and Patricia C. Zambryski},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1987},
  volume={84 17},
  pages={6169-73}
}
The transfer DNA (T-DNA) mobilized into plant cells by Agrobacterium tumefaciens seems to integrate rather randomly into the plant genome. We analyzed a target site in the genome of Nicotiana tabacum before and after integration of a T-DNA. Clones presenting right and left T-DNA/plant DNA junctions were used as probes to identify and isolate a unique 1.8-kilobase EcoRI fragment corresponding to the plant DNA target site for a T-DNA insertion event. Comparison of the nucleotide sequences of the… CONTINUE READING