Integration and global analysis of isothermal titration calorimetry data for studying macromolecular interactions

@article{Brautigam2016IntegrationAG,
  title={Integration and global analysis of isothermal titration calorimetry data for studying macromolecular interactions},
  author={Chad A. Brautigam and Huaying Zhao and Carolyn Vargas and Sandro Keller and Peter Schuck},
  journal={Nature Protocols},
  year={2016},
  volume={11},
  pages={882-894}
}
Isothermal titration calorimetry (ITC) is a powerful and widely used method to measure the energetics of macromolecular interactions by recording a thermogram of differential heating power during a titration. However, traditional ITC analysis is limited by stochastic thermogram noise and by the limited information content of a single titration experiment. Here we present a protocol for bias-free thermogram integration based on automated shape analysis of the injection peaks, followed by… CONTINUE READING

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