Integration, expression and inheritance of transgenes in hexaploid oat (Avena sativa L.).

@article{Perret2003IntegrationEA,
  title={Integration, expression and inheritance of transgenes in hexaploid oat (Avena sativa L.).},
  author={Sophie J Perret and John W Valentine and Jeannie Leggett and Phillip Morris},
  journal={Journal of plant physiology},
  year={2003},
  volume={160 8},
  pages={931-43}
}
Two oat varieties, Melys (spring variety) and Bulwark (winter variety) were transformed by particle bombardment of primary embryogenic callus using either a ubi-bar-ubi-gus co-integration vector or co-transformed (Melys) with a ubi-bar plasmid together with one of three plasmids containing the beta-glucuronidase (gus) gene under the control of either a rice actin promoter, a CaMV35S promoter or a wheat high molecular weight glutenin promoter. Morphologically normal and fertile transgenic plants… CONTINUE READING