Integrated platform of capillary isoelectric focusing, trypsin immobilized enzyme microreactor and nanoreversed-phase liquid chromatography with mass spectrometry for online protein profiling.

Abstract

An integrated platform with the combination of protein and peptide separation was established via online protein digestion, by which proteins were first separated by CIEF, online digested by a trypsin immobilized enzyme microreactor, trapped and desalted by two parallel trap columns, separated by nanoreversed-phase and finally identified by MS. In such a… (More)
DOI: 10.1002/elps.201100030

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@article{Wang2011IntegratedPO, title={Integrated platform of capillary isoelectric focusing, trypsin immobilized enzyme microreactor and nanoreversed-phase liquid chromatography with mass spectrometry for online protein profiling.}, author={Tingting Wang and Junfeng Ma and Shuaibin Wu and Huiming Yuan and Lihua Zhang and Zhen Liang and Yukui Zhang}, journal={Electrophoresis}, year={2011}, volume={32 20}, pages={2848-56} }