Integrated photothermal flow cytometry in vivo.

@article{Zharov2005IntegratedPF,
  title={Integrated photothermal flow cytometry in vivo.},
  author={Vladimir P. Zharov and Ekaterina I Galanzha and Valery V. Tuchin},
  journal={Journal of biomedical optics},
  year={2005},
  volume={10 5},
  pages={
          051502
        }
}
The capability of integrated flow cytometry to detect, in real time, moving cells in their natural states in vivo is demonstrated in a study of circulating red and white blood cells in lymph and blood flow of rat mesentery. This system combines dual pump-probe photothermal (PT) techniques, such as PT imaging, the PT thermolens method, and PT velocimetry, with high-resolution (up to 0.3 microm), high-speed (up to 1000 fps) transmission digital microscopy (TDM) and fluorescence imaging. All PT… 
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37 Citations

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TLDR
Using animal mesentery with intravital optical microscopy is a well-established experimental model for studying blood and lymph microcirculation in vivo and demonstrated that individual cell transportation in living organisms depends on cell type, the cellos functional state, and the functional status of the organism.
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A combination of high resolution PT imaging with PT spectral identification and ultrasensitive quantitative PT characterization of cytochrome c within individual mitochondria in single live cells is demonstrated for the first time.
In vivo photoacoustic and photothermal cytometry for monitoring multiple blood rheology parameters
  • E. Galanzha, V. Zharov
  • Biology, Medicine
    Cytometry. Part A : the journal of the International Society for Analytical Cytology
  • 2011
TLDR
The multi‐parameter platform that integrates PT, PA, and conventional optical techniques has potential for translation to clinical applications using safe, portable, laser‐based medical devices for point‐of‐care screening of disease progression and therapy efficiency.
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