Integrated bombardment andAgrobacterium transformation system: An alternative method for soybean transformation

@article{Droste2008IntegratedBA,
  title={Integrated bombardment andAgrobacterium transformation system: An alternative method for soybean transformation},
  author={A. Droste and G. Pasquali and M. H. Bodanese-Zanettini},
  journal={Plant Molecular Biology Reporter},
  year={2008},
  volume={18},
  pages={51-59}
}
  • A. Droste, G. Pasquali, M. H. Bodanese-Zanettini
  • Published 2008
  • Biology
  • Plant Molecular Biology Reporter
  • Described here is a new method for soybean transformation, based on microwounding of embryonic clumps by particle bombardment prior to inoculation with anAgrobacterium suspension. The method combines the advantages of somatic embryogenesis and gene transfer through an integrated transformation system. This is the first report of application of this technique to transformation ofGlycine max. 
    33 Citations

    Figures from this paper.

    Cultivo de tejidos y transformación genética en Glycine max (L.) Merrill
    A transgenic tropical maize line generated by the direct transformation of the embryo-scutellum by A. tumefaciens
    • 35
    Efficient particle bombardment-mediated transformation of Cuban soybean (INCASoy-36) using glyphosate as a selective agent
    • 6
    • PDF

    References

    SHOWING 1-10 OF 30 REFERENCES
    Transformation of cotton (Gossypium hirsutum L.) via particle bombardment
    • 220
    Induction and in vitro culture of soybean crown gall tumors
    • 39
    Recovery of primary transformants of soybean
    • 124
    • PDF
    Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA.
    • 2,994
    • Highly Influential
    • PDF
    Microprojectile bombardment of plant tissues increases transformation frequency by Agrobacterium tumefaciens
    • 126
    Transformation of 12 different plasmids into soybean via particle bombardment
    • 153
    Production of transgenic soybean lines expressing the bean pod mottle virus coat protein precursor gene
    • 119
    Strain and cultivar specificity in the Agrobacterium-soybean interaction
    • 45