Integrated RNA extraction and RT-PCR for semi-quantitative gene expression studies on a microfluidic device


This paper describes the development of a microfluidic methodology, using RNA extraction and reverse transcription PCR, for investigating expression levels of cytochrome P450 genes. Cytochrome P450 enzymes are involved in the metabolism of xenobiotics, including many commonly prescribed drugs, therefore information on their expression is useful in both… (More)
DOI: 10.1038/labinvest.2013.76


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