Integrated Approach To Producing High-Purity Trehalose from Maltose by the Yeast Yarrowia lipolytica Displaying Trehalose Synthase (TreS) on the Cell Surface.

@article{Li2016IntegratedAT,
  title={Integrated Approach To Producing High-Purity Trehalose from Maltose by the Yeast Yarrowia lipolytica Displaying Trehalose Synthase (TreS) on the Cell Surface.},
  author={Ning Li and Hengwei Wang and Lijuan Li and Huiling Cheng and Dawen Liu and Hairong Cheng and Zixin Deng},
  journal={Journal of agricultural and food chemistry},
  year={2016},
  volume={64 31},
  pages={
          6179-87
        }
}
An alternative strategy that integrated enzyme production, trehalose biotransformation, and bioremoval in one bioreactor was developed in this study, thus simplifying the traditional procedures used for trehalose production. The trehalose synthase gene from a thermophilic archaea, Picrophilus torridus, was first fused to the YlPir1 anchor gene and then inserted into the genome of Yarrowia lipolytica, thus yielding an engineered yeast strain. The trehalose yield reached 73% under optimal… 

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References

SHOWING 1-10 OF 47 REFERENCES
Biocatalytic Production of Trehalose from Maltose by Using Whole Cells of Permeabilized Recombinant Escherichia coli
TLDR
The biocatalytic process developed in this study might serve as a candidate for the large scale production of trehalose.
Overexpression and characterization of a thermostable trehalose synthase from Meiothermus ruber
TLDR
A thermostable trehalose synthase (TreS) gene from Meiothermus ruber CBS-01 was cloned and overexpressed in Escherichia coli and showed an optimum pH and temperature, indicating maltose as the preferred substrate.
Gene cloning and characterization of a trehalose synthase from Corynebacterium glutamicum ATCC13032
TLDR
A gene (cg2529) encoding for TreS from Corynebacterium glutamicum was cloned and expressed in Escherichia coli, and the maximum conversion yield by CgTS reached 69% at 25°C after 9 hr of reaction, leading to increase in the conversion yields from maltose into trehalose.
Simultaneous production of trehalose, bioethanol, and high-protein product from rice by an enzymatic process.
TLDR
An efficient enzymatic procedure has been successfully developed to simultaneously produce value-added trehalose, bioethanol, and high-protein product from rice as substrate and the conversion yield was about 98% after 64 h of fermentation time by Saccharomyces cerevisiae without any artificial culture solution addition.
Identification and Characterization of a Novel Trehalose Synthase Gene Derived from Saline-Alkali Soil Metagenomes
TLDR
The maximum conversion rate of maltose into trehalose by the TreS was reached more than 78% at a relatively high maltose concentration, making it a good candidate in the large-scale production of trehalsoe after further study.
Gene cloning, expression, and biochemical characterization of a recombinant trehalose synthase from Picrophilus torridus in Escherichia coli.
TLDR
Kinetic analysis showed that the enzyme has a 2.5-fold higher catalytic efficiency for maltose than for trehalose, indicating maltose as the preferred substrate, and five amino acid residues that are important for alpha-amylase family enzyme catalysis were shown to be conserved in PTTS.
An Alternative Approach to Synthesizing Galactooligosaccharides by Cell-Surface Display of β-Galactosidase on Yarrowia lipolytica.
TLDR
An alternative strategy for synthesizing galactooligosaccharides (GOS) from an erythritol-producing yeast Yarrowia lipolytica using surface display technology was demonstrated and the optimal reaction temperature increased to 60 °C, which was helpful for enhancing the reaction rate and GOS production.
A novel thermophilic fusion enzyme for trehalose production
TLDR
It is demonstrated that the bifunctional fusion enzyme is able to produce trehalose starting from malto-oligosaccharides at 75°C and partially purified the recombinant fusion protein from bacterial cell free extracts and from insoluble fractions in which the fusion protein was also found as aggregate in inclusion bodies.
Gene cloning, expression, and characterization of a novel trehalose synthase from Arthrobacter aurescens
TLDR
A novel treS gene, which had a length of 1,797 bp and encoded 598 amino acids, was cloned from Arthrobacter aurescens CGMCC 1.1892 and expressed in Escherichia coli and Thin layer chromatography results indicated that it could catalyze the conversion between maltose and trehalose in one step.
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3
4
5
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