Integral refractive index imaging of flowing cell nuclei using quantitative phase microscopy combined with fluorescence microscopy.

  title={Integral refractive index imaging of flowing cell nuclei using quantitative phase microscopy combined with fluorescence microscopy.},
  author={Gili Dardikman and Yoav N. Nygate and Itay Barnea and Nir A. Turko and Gyanendra Singh and Barham Javidi and Natan T. Shaked},
  journal={Biomedical optics express},
  volume={9 3},
We suggest a new multimodal imaging technique for quantitatively measuring the integral (thickness-average) refractive index of the nuclei of live biological cells in suspension. For this aim, we combined quantitative phase microscopy with simultaneous 2-D fluorescence microscopy. We used 2-D fluorescence microscopy to localize the nucleus inside the quantitative phase map of the cell, as well as for measuring the nucleus radii. As verified offline by both 3-D confocal fluorescence microscopy… 

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