Integral and peripheral protein composition of the apical and basolateral membrane domains in MDCK cells

@article{Sargiacomo1989IntegralAP,
  title={Integral and peripheral protein composition of the apical and basolateral membrane domains in MDCK cells},
  author={Massimo Sargiacomo and Michael Phillip Lisanti and Lutz Graeve and Andr{\'e} Le Bivic and Enrique Rodr{\'i}guez-Boulan},
  journal={The Journal of Membrane Biology},
  year={1989},
  volume={107},
  pages={277-286}
}
Selective biotinylation of the apical or basolateral domains of confluent MDCK monolayers grown on polycarbonate filters with a water soluble biotin analog, sulfo-NHS-biotin, was employed to reveal strikingly distinct patterns of endogenous “peripheral” and “integral” membrane proteins. “Peripheral” proteins were found to be approximately fivefold more abundant with this procedure than “integral” membrane proteins, both on the apical and on the basolateral surface. The distinct apical and basal… CONTINUE READING
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A water-soluble biotin reagent as a tool for labeling polarized MDCK plasma membrane proteins

  • M. Sargiacomo, L. Graeve, E. Rodriguez-Boulan
  • J. Cell Biol
  • 1987

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