Intake of fermented soybean (natto) increases circulating vitamin K2 (menaquinone-7) and γ-carboxylated osteocalcin concentration in normal individuals

  title={Intake of fermented soybean (natto) increases circulating vitamin K2 (menaquinone-7) and $\gamma$-carboxylated osteocalcin concentration in normal individuals},
  author={Yoshinori Tsukamoto and Hideyuki Ichise and Hiroyuki Kakuda and Masayoshi Yamaguchi},
  journal={Journal of Bone and Mineral Metabolism},
Abstract: Changes in circulating vitamin K2 (menaquinone-7, MK-7) and γ-carboxylated osteocalcin concentrations in normal individuals with the intake of fermented soybeans (natto) were investigated. [] Key Method Eight male volunteers were given sequentially fermented soybeans (natto) containing three different contents of MK-7 at an interval of 7 days as follows: regular natto including 775 μg/100 g (MK-7 × 1) or reinforced natto containing 1298 μg/100 g (MK-7 × 1.5) or 1765 μg/100 g (MK-7 × 2…

Low-dose menaquinone-7 supplementation improved extra-hepatic vitamin K status, but had no effect on thrombin generation in healthy subjects

MK-7 supplementation at doses in the order of the RDA (Commission Directive 2008/100/EC) increased the carboxylation of circulating OC and MGP, and no adverse effects on thrombin generation were observed.

Vitamin K2 (menaquinone 4) reduces serum undercarboxylated osteocalcin level as early as 2 weeks in elderly women with established osteoporosis

In this randomized prospective study, the MK4 group shows a reduction in the serum uc-OC level within 2 weeks without any significant change in OC, suggesting that the uc -OC is changed to carboxylated OC.

The antiosteoporotic effects of Cheonggukjang containing vitamin k2 (menaquinone-7) in ovariectomized rats.

Results showed that rats treated with a high dose of MK-7 (8 μg/day) exhibited a minor inhibitory effect on OVX-induced bone loss, as indicated by a significant increase in trabecular number, as well as BMC and BMD (P<.01).

Stimulatory effect of menaquinone-7 (vitamim K2) on osteoblastic bone formation in vitro

It is demonstrated that MK-7 has an anabolic effect on bone tissue and osteoblastic MC3T3-E1 cells in vitro, suggesting that the compound can stimulate osteoblastics bone formation.

The association between dietary vitamin K intake and serum undercarboxylated osteocalcin is modulated by vitamin K epoxide reductase genotype

The results show that the inverse association between dietary vitamin K intake and serum ucOC depends on a functionally relevant allelic variant of the VKORC1 gene.

Inhibitory effect of menaquinone-7 (vitamin K2) on the bone-resorbing factors-induced bone resorption in elderly female rat femoral tissues in vitro

The view that MK-7 has a direct inhibitory effect on the bone-resorbing factor-induced bone resorption in bone culture using female aged femoral tissues in vitro is supported.

Effect of low dose vitamin K2 (MK-4) supplementation on bio-indices in postmenopausal Japanese women.

Results suggest that supplementation with 1.5 mg/d MK-4 accelerated the degree of OC gamma-carboxylation and might be beneficial in the maintenance of bone health in postmenopausal Japanese women.

Promotion of bone formation by fermented soybean (Natto) intake in premenopausal women.

In premenopausal women who had to keep the stiffness index as high as possible before menopause, Natto intake may have contributed to the promotion of bone formation.

Inhibitory effect of menaquinone-7 (vitamin K2) on osteoclast-like cell formation and osteoclastic bone resorption in rat bone tissues in vitro

It is demonstrated that menaquinone-7 has an inhibitory effect on osteoclastic bone resorption in vitro and the inhibitory action of MK-7 is partly involved in protein kinase C signaling.

The role of menaquinones (vitamin K₂) in human health.

It is concluded that further investigations are needed to establish how differences among the vitamin K forms may influence tissue specificities and their role in human health, and there is merit for considering both menaquinones and phylloquinone when developing future recommendations for vitamin K intake.



Prolonged intake of fermented soybean (natto) diets containing vitamin K2 (menaquinone-7) prevents bone loss in ovariectomized rats

It is demonstrated that the prolonged intake of natto dietary including MK-7 has a preventive effect on bone loss induced by OVX, and may be useful in the prevention of osteoporosis.

Effect of vitamin K2 (menaquinone-7) in fermented soybean (natto) on bone loss in ovariectomized rats

It is demonstrated that the intake of dietary MK-7 has a preventive effect on bone loss caused by OVX, which may be partly caused by MK-4, which is formed by degradation ofMK-7.

Effect of vitamin K2 (menaquinone-7) on bone metabolism in the femoral-metaphyseal tissues of normal and skeletal-unloaded rats: enhancement with zinc

It is demonstrated that the effect of vitamin K2 (menaquinone-7) on trabecular bone calcification in rats with skeletal unloading-induced osteopenia is enhanced by zinc in vitro, which may be based on a newly synthesized protein in the bone tissues.

Vitamin K-dependent formation of bone Gla protein (osteocalcin) and its function.

  • P. Price
  • Biology, Chemistry
    Vitamins and hormones
  • 1985

Vitamin K intake and hip fractures in women: a prospective study.

The hypothesis that high intakes of vitamin K are associated with a lower risk of hip fracture in women is investigated and the suggestion for a reassessment of the vitamin K requirements that are based on bone health and blood coagulation is supported.

A one step sandwich enzyme immunoassay for gamma-carboxylated osteocalcin using monoclonal antibodies.

Undercarboxylated osteocalcin measured with a specific immunoassay predicts hip fracture in elderly women: the EPIDOS Study.

It is found that ucOC, but not total OC, predicts hip fracture risk independently of femoral neck BMD in elderly women drawn from the general population, with low cross-reactivity with carboxylated OC and increased specificity and sensitivity over the HAP assay.

Calcium-dependent alpha-helical structure in osteocalcin.

Helical osteocalcin has greatly increased affinity for HA; thus, the Ca2+-induced structural transition may perform an informational role related to bone metabolism.