Intact protein analysis of ubiquitin in cerebrospinal fluid by multiple reaction monitoring reveals differences in Alzheimer's disease and frontotemporal lobar degeneration.

@article{Oeckl2014IntactPA,
  title={Intact protein analysis of ubiquitin in cerebrospinal fluid by multiple reaction monitoring reveals differences in Alzheimer's disease and frontotemporal lobar degeneration.},
  author={Patrick Oeckl and Petra Steinacker and Christine A. F. von Arnim and Sarah Straub and Magdalena Nagl and Emily Feneberg and Jochen H Weishaupt and Albert Christian Ludolph and Markus Otto},
  journal={Journal of proteome research},
  year={2014},
  volume={13 11},
  pages={
          4518-25
        }
}
The impairment of the ubiquitin-proteasome system (UPS) is thought to be an early event in neurodegeneration, and monitoring UPS alterations might serve as a disease biomarker. Our aim was to establish an alternate method to antibody-based assays for the selective measurement of free monoubiquitin in cerebrospinal fluid (CSF). Free monoubiquitin was measured with liquid chromatography-multiple reaction monitoring mass spectrometry (LC-MS/MS) in CSF of patients with Alzheimer's disease (AD… 
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