Intact protein analysis by matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry.

@article{Lin2003IntactPA,
  title={Intact protein analysis by matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry.},
  author={Melanie Lin and Jennifer M Campbell and Dieter R Mueller and Urs Wirth},
  journal={Rapid communications in mass spectrometry : RCM},
  year={2003},
  volume={17 16},
  pages={1809-14}
}
Direct tandem mass spectrometric (MS/MS) analysis of small, singly charged protein ions by tandem time-of-flight mass spectrometry (TOFMS) is demonstrated for proteins up to a molecular mass of 12 kDa. The MALDI-generated singly charged precursor ions predominantly yield product ions resulting from metastable fragmentation at aspartyl and prolyl residues. Additional series of C-terminal sequence ions provide in some cases sufficient information for protein identification. The amount of sample… CONTINUE READING

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