Insulin unmasks a COOH-terminal Glut4 epitope and increases glucose transport across T-tubules in skeletal muscle

@article{Wang1996InsulinUA,
  title={Insulin unmasks a COOH-terminal Glut4 epitope and increases glucose transport across T-tubules in skeletal muscle},
  author={Wei Wang and Polly A Hansen and Bess Adkins Marshall and John O. Holloszy and Mike M Mueckler},
  journal={The Journal of Cell Biology},
  year={1996},
  volume={135},
  pages={415 - 430}
}
An improved immunogold labeling procedure was used to examine the subcellular distribution of glucose transporters in Lowricryl HM20-embedded skeletal muscle from transgenic mice overexpressing either Glut1 or Glut4. In basal muscle, Glut4 was highly enriched in membranes of the transverse tubules and the terminal cisternae of the triadic junctions. Less than 10% of total muscle Glut4 was present in the vicinity of the sarcolemmal membrane. Insulin treatment increased the number of gold… CONTINUE READING