Insulin stimulates the activity of a protamine kinase in isolated rat hepatocytes.

@article{Reddy1990InsulinST,
  title={Insulin stimulates the activity of a protamine kinase in isolated rat hepatocytes.},
  author={Smitha A. L. Narasimha Reddy and Grayson D. Amick and R. H. Cooper and Zahi Damuni},
  journal={The Journal of biological chemistry},
  year={1990},
  volume={265 14},
  pages={
          7748-52
        }
}
Treatment of isolated rat hepatocytes with 10-100 nM insulin for 5-10 min increased by about 2-fold the activity of a protamine kinase which exhibited properties similar to those of a protamine kinase from bovine kidney (Damuni, Z., Amick, G. D., and Sneed, T. R. (1989) J. Biol. Chem. 264, 6412-6416). Half-maximal increase in protamine kinase activity occurred at about 1 nM insulin. This effect of insulin was detected only when 25 mM NaF or 50 mM KPO4 were included in the homogenization buffers… CONTINUE READING