Insulin secretion in aging: studies with sequential gating of secretion vesicle margination and lysis.

Abstract

A new sequential gating perifusion technique was employed to investigate secretion vesicle margination and granule lysis in islets isolated from 2- and 18-month-old Fischer 344 rats. The technique is based on sequential perifusion (periods A, B, and C) of isolated islets with glucose (30, 165, or 300 mg/dl) in the presence of sodium isethionate, an… (More)

Topics

  • Presentations referencing similar topics