Insulin-like growth factor I in cats: validation of an enzyme-linked immunosorbent assay and determination of biologic variation.

@article{Strage2015InsulinlikeGF,
  title={Insulin-like growth factor I in cats: validation of an enzyme-linked immunosorbent assay and determination of biologic variation.},
  author={Emma M Strage and Elvar Theodorsson and Bodil Str{\"o}m Holst and Inger E Lillieh{\"o}{\"o}k and Moira S. Lewitt},
  journal={Veterinary clinical pathology},
  year={2015},
  volume={44 4},
  pages={542-51}
}
BACKGROUND Insulin-like growth factor I (IGF-I) measurements are used in veterinary medicine for diagnosing growth hormone disorders. IGF-I assays are subject to interference by IGF-binding proteins (IGFBP) which may not be efficiently removed by standard extraction methods. Adding excess IGF-II during analysis may improve accuracy. OBJECTIVES The purpose of the study was to validate a commercial human IGF-I ELISA which uses excess IGF-II for feline samples and to evaluate biologic variation… CONTINUE READING