Insulin-like growth factor-I and slow, bi-directional perfusion enhance the formation of tissue-engineered cardiac grafts.

@article{Cheng2009InsulinlikeGF,
  title={Insulin-like growth factor-I and slow, bi-directional perfusion enhance the formation of tissue-engineered cardiac grafts.},
  author={Mingyu Cheng and Matteo Moretti and George C. Engelmayr and Lisa E. Freed},
  journal={Tissue engineering. Part A},
  year={2009},
  volume={15 3},
  pages={
          645-53
        }
}
Biochemical and mechanical signals enabling cardiac regeneration can be elucidated using in vitro tissue-engineering models. We hypothesized that insulin-like growth factor-I (IGF) and slow, bi-directional perfusion could act independently and interactively to enhance the survival, differentiation, and contractile performance of tissue-engineered cardiac grafts. Heart cells were cultured on three-dimensional porous scaffolds in medium with or without supplemental IGF and in the presence or… Expand
Biomimetic scaffold combined with electrical stimulation and growth factor promotes tissue engineered cardiac development.
TLDR
This culture environment, designed to combine cardiac-like scaffold architecture and biomechanics with molecular and biophysical signals, enabled functional assembly of engineered heart muscle from dissociated cells and could serve as a template for future studies on the hierarchy of various signaling domains relative to cardiac tissue development. Expand
Enhancement of adipose-derived stem cell differentiation in scaffolds with IGF-I gene impregnation under dynamic microenvironment.
Biochemical and mechanical signals enabling cardiac regeneration can be elucidated by using in vitro tissue engineering models. We hypothesized that human insulin-like growth factor-I (IGF-I) andExpand
Effects of mechanical stimulation induced by compression and medium perfusion on cardiac tissue engineering
TLDR
The formation of an improved cardiac tissue in vitro is established, when induced by combined mechanical signals of compression and fluid shear stress provided by perfusion. Expand
Activation of the ERK1/2 cascade via pulsatile interstitial fluid flow promotes cardiac tissue assembly.
TLDR
It is demonstrated that by judiciously applying fluid shear stress, cell signaling cascades can be augmented with subsequent profound effects on cardiac tissue regeneration, as compared to static-cultivated constructs. Expand
Dynamic culture yields engineered myocardium with near-adult functional output.
TLDR
A versatile methodology for engineering cardiac tissues with a near-adult functional output without the need for exogenous electrical or mechanical stimulation is developed, and mTOR signaling is identified as an important mechanism for advancing tissue maturation and function in vitro. Expand
Engineering Functional Tissues: In Vitro Culture Parameters
TLDR
Representative studies of two diverse types of engineered tissues – cartilage and cardiac – are described in which in vitro culture parameters were manipulated in order to accelerate the growth, maturation and integration of engineered tissue constructs and improve their biomechanical properties. Expand
The role of mechanical forces in cardiomyocyte differentiation in 3D culture
TLDR
3D collagen gel bioreactor culture, with capabilities for spatial and temporal monitoring, represents a powerful model for elucidating the role of specific environmental factors and their underlying mechanisms on directed cell proliferation and differentiation. Expand
Biomimetic perfusion and electrical stimulation applied in concert improved the assembly of engineered cardiac tissue
TLDR
The simultaneous application of medium perfusion and electrical conditioning enabled by the use of the novel bioreactor system may accelerate the generation of fully functional, clinically sized cardiac tissue constructs. Expand
Fabrication of electrospun poly (lactide-co-glycolide)-fibrin multiscale scaffold for myocardial regeneration in vitro.
TLDR
The results indicate that this fibrin-based multiscale electrospun composite scaffold enhances the differentiation of mesenchymal stem cells into cardiomyocytes and demonstrates the promising potential of this scaffold for myocardial tissue engineering applications. Expand
Engineered Macroscale Cardiac Constructs Elicit Human Myocardial Tissue-like Functionality
Summary In vitro surrogate models of human cardiac tissue hold great promise in disease modeling, cardiotoxicity testing, and future applications in regenerative medicine. However, the generation ofExpand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 54 REFERENCES
Effects of regulatory factors on engineered cardiac tissue in vitro.
TLDR
The results demonstrate that supplemental regulatory molecules can differentially enhance properties of cardiac tissue constructs and imply that these constructs can provide a platform for systematic in vitro studies of the effects of complex stimuli that occur in vivo to improve the basic understanding of cardiogenesis. Expand
Enhancement of adipose-derived stem cell differentiation in scaffolds with IGF-I gene impregnation under dynamic microenvironment.
Biochemical and mechanical signals enabling cardiac regeneration can be elucidated by using in vitro tissue engineering models. We hypothesized that human insulin-like growth factor-I (IGF-I) andExpand
Medium perfusion enables engineering of compact and contractile cardiac tissue.
TLDR
An in vitro culture system that maintains efficient oxygen supply to the cells at all times during cell seeding and construct cultivation and characterized in detail construct metabolism, structure, and function is designed. Expand
Effects of oxygen on engineered cardiac muscle.
TLDR
The control of oxygen concentration in cell microenvironment can improve the structure and function of engineered cardiac muscle and form a basis for controlled studies of the effects of oxygen on the in vitro development of engineered tissues. Expand
Differentiation Enhancement of ADSC in Scaffolds With IGF-1 Gene Impregnation Under Dynamic Microenvironment
TLDR
It was hypothesized that human insulin-like growth factor-1 (IGF-1) and three dimensional dynamic microenvironment could act independently and interactively to enhance the survival and differentiation of adipose tissue-derived stem cells (ADSCs) and hence the construction of engineered cardiac grafts. Expand
Local myocardial insulin-like growth factor 1 (IGF-1) delivery with biotinylated peptide nanofibers improves cell therapy for myocardial infarction.
TLDR
Self-assembling peptide nanofibers for prolonged delivery of insulin-like growth factor 1 (IGF-1), a cardiomyocyte growth and differentiation factor, to the myocardium using a "biotin sandwich" approach improved systolic function after experimental myocardial infarction, demonstrating how engineering the local cellular microenvironment can improve cell therapy. Expand
Perfusion improves tissue architecture of engineered cardiac muscle.
TLDR
Medium perfusion could be utilized to better mimic the transport conditions within native cardiac muscle and enable in vitro engineering of cardiac constructs with clinically useful thicknesses. Expand
High-density seeding of myocyte cells for cardiac tissue engineering.
TLDR
Direct perfusion can enable seeding of hypoxia-sensitive cells at physiologically high and spatially uniform initial densities and maintain cell viability and function. Expand
Chronic Pulsatile Shear Stress Alters Insulin-Like Growth Factor-I (IGF-I) Binding Protein Release In Vitro
TLDR
It is suggested that shear stress may indirectly regulate IGF-I activity, and, by extension, the effect of IGF-i on vascular pathologies, as well as how shear impacts the IGF axis. Expand
Cardiac tissue engineering: cell seeding, cultivation parameters, and tissue construct characterization.
TLDR
Advantages of culturing constructs under mixed rather than static conditions included the maintenance of metabolic parameters in physiological ranges, 2-4 times higher construct cellularity, more aerobic cell metabolism, and a more physiological, elongated cell shape. Expand
...
1
2
3
4
5
...