Insulin-like growth factor-1, a component of serum that enables porcine cumulus cells to expand in response to follicle-stimulating hormone in vitro.

@article{Singh1997InsulinlikeGF,
  title={Insulin-like growth factor-1, a component of serum that enables porcine cumulus cells to expand in response to follicle-stimulating hormone in vitro.},
  author={Bhagirath Singh and David T Armstrong},
  journal={Biology of reproduction},
  year={1997},
  volume={56 6},
  pages={1370-5}
}
The present study was aimed at determining whether insulin-like growth factor-1 (IGF-1) is a component of fetal bovine serum (FBS) that enables porcine cumulus cells to expand in response to FSH in vitro. Cumulus-oocyte complexes (COC) obtained from 4- to 6-mm follicles of prepubertal gilts were cultured at 39 degrees C for 24 h in media that contained human recombinant IGF-1 (50 ng/ml), FBS (15% v:v), or their combination, with or without FSH (1.5 microg/ml), and cumulus expansion was scored… CONTINUE READING

5 Figures & Tables

Connections & Topics

Mentioned Connections BETA
OocytesAnatomic structure is physical part ofOvarian Follicle
Cumulus - oocyte complexes ( COC ) obtained from 4- to 6-mm follicles of prepubertal gilts were cultured at 39 degrees C for 24 h in media that contained human recombinant IGF-1 ( 50 ng / ml ) , FBS ( 15% v : v ) , or their combination , with or without FSH ( 1.5 microg / ml ) , and cumulus expansion was scored microscopically .
All Topics