Insulin gene expression and insulin synthesis in mammalian neuronal cells.

@article{Devaskar1994InsulinGE,
  title={Insulin gene expression and insulin synthesis in mammalian neuronal cells.},
  author={Sherin U. Devaskar and Stephen J. Giddings and Premeela A. Rajakumar and Lynn R. Carnaghi and Ram Kumar Menon and Daniel S Zahm},
  journal={The Journal of biological chemistry},
  year={1994},
  volume={269 11},
  pages={
          8445-54
        }
}
To demonstrate the presence of de novo synthesis in central mammalian neurons, we cloned and sequenced a rabbit insulin cDNA from pancreas and used it to define sequences encoding insulin mRNA from postnatal rabbit brain. We observed transcription/elongation of nascent insulin transcripts, characterized the size of these transcripts, and localized them to specific neurons in certain catecholaminergic-rich areas of the central nervous system. RNase protection assays using a rabbit probe spanning… Expand
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