Insulating behavior of lambda-DNA on the micron scale.

  title={Insulating behavior of lambda-DNA on the micron scale.},
  author={Y. Zhang and Robert H. Austin and Jessica J. Kraeft and Edward C. Cox and Nai Phuan Ong},
  journal={Physical review letters},
  volume={89 19},
We have investigated the electrical conductivity of lambda-DNA using DNA covalently bonded to Au electrodes. Thiol-modified dTTP was incorporated into the "sticky" ends of bacteriophage lambda-DNA using DNA polymerase. Two-probe measurements on such molecules provide a hard lower bound for the resistivity rho>10(6)Omega cm at bias potentials up to 20 V, in conflict with recent claims of moderate to high conductivity. By direct imaging, we show that the molecules are present after the… 

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Nature 398

  • 407
  • 1999

European J

  • Biochem. 3, 85 (1967); T. V. S. Rao et al., Bioorg. Med. Chem. Lett. 10, 907
  • 2000

1×TE buffer contains 10 mM Tris (tris(hydroxymethyl)aminomethane ) and 1 mM EDTA (ethylene diamine tetra acetic acid), pH 8

  • 1×TE buffer contains 10 mM Tris (tris(hydroxymethyl)aminomethane ) and 1 mM EDTA (ethylene diamine tetra acetic acid), pH 8

Proc. Natl. Acad. Sci. USA 99

  • Proc. Natl. Acad. Sci. USA 99
  • 2002

Trans. Faraday Soc

  • Trans. Faraday Soc
  • 1962

Phys. Rev. Lett

  • Phys. Rev. Lett
  • 2000

Appl. Phys. Lett

  • Appl. Phys. Lett
  • 2001

Nano Lett

  • Nano Lett
  • 2002