Insulated Isothermal Reverse Transcriptase PCR (iiRT-PCR) for Rapid and Sensitive Detection of Classical Swine Fever Virus.

@article{Lung2016InsulatedIR,
  title={Insulated Isothermal Reverse Transcriptase PCR (iiRT-PCR) for Rapid and Sensitive Detection of Classical Swine Fever Virus.},
  author={O Lung and J Pasick and Mathew C. Fisher and Cody Buchanan and Alan Erickson and Aruna Ambagala},
  journal={Transboundary and emerging diseases},
  year={2016},
  volume={63 5},
  pages={e395-402}
}
Classical swine fever (CSF) is an OIE-listed disease that can have a severe impact on the swine industry. User-friendly, sensitive, rapid diagnostic tests that utilize low-cost field-deployable instruments for CSF diagnosis can be useful for disease surveillance and outbreak monitoring. In this study, we describe validation of a new probe-based insulated isothermal reverse transcriptase PCR (iiRT-PCR) assay for rapid detection of classical swine fever virus (CSFV) on a compact, user-friendly… CONTINUE READING
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