Instrumental assay of microbial lipase at constant pH.

  title={Instrumental assay of microbial lipase at constant pH.},
  author={C L San Clemente and D. V. Vadehra},
  journal={Applied microbiology},
  volume={15 1},
A rapid, accurate method with high sensitivity and reproducibility, and having the advantage of a short incubation period under constant pH, has been developed for routine measurement of microbial lipase. Assembled from readily available and economical instrumental components, the apparatus includes a pH meter, a thermoelectric heating and stirring device, a motor-driven burette, and an automatic recorder. The reaction mixture, consisting of 5 ml of a 10% olive oil-gum arabic emulsion, 2 ml of… CONTINUE READING