Instability of, and generation of hydrogen peroxide by, phenolic compounds in cell culture media.

@article{Long2010InstabilityOA,
  title={Instability of, and generation of hydrogen peroxide by, phenolic compounds in cell culture media.},
  author={Lee Hua Long and Aina Hoi and Barry Halliwell},
  journal={Archives of biochemistry and biophysics},
  year={2010},
  volume={501 1},
  pages={162-9}
}
Many papers in the literature have described complex effects of flavonoids and other polyphenols on cells in culture. In this paper we show that hydroxytyrosol, delphinidin chloride and rosmarinic acid are unstable in three commonly-used cell culture media (Dulbecco's modified Eagle's medium (DMEM), RPMI 1640 (RPMI) and Minimal Essential Medium Eagle (MEM)) and undergo rapid oxidation to generate H2O2. This may have confounded some previous studies on the cellular effects of these compounds. By… CONTINUE READING