Salutaridine reductase (NADPH) is a key enzyme involves in morphine biosynthesis. BLASTP search waer performed to found orthologous proteins across different plant species. Domain analysis of all orthologous proteins was performed using Pfam and it was observed that Adh_short domain was conserved across all proteins. 3D structure of Salutaridine reductase was not available in PDB database, therefore Homology model of Salutaridine reductase was constructed using HOMER. A template structure (PDB ID: 2PFG) was selected from protein databank (PDB) using BLASTP with BLOSUM62 sequence alignment scoring matrix. The protein model was validated using PROCHECK. The model structure was visualized and superimposed with template using Discovery Studio Visualizer (Accelrys) and Swiss PDBviewer.