Insights into the phylogeny and coding potential of microbial dark matter

@article{Rinke2013InsightsIT,
  title={Insights into the phylogeny and coding potential of microbial dark matter},
  author={Christian Rinke and Patrick Schwientek and Alexander Sczyrba and Natalia N. Ivanova and Iain Anderson and Jan-Fang Cheng and Aaron E. Darling and Stephanie Malfatti and Brandon K. Swan and Esther A. Gies and Jeremy A. Dodsworth and Brian P. Hedlund and George Tsiamis and Stefan M. Sievert and Wen-Tso Liu and Jonathan A. Eisen and Steven J. Hallam and Nikos C. Kyrpides and Ramunas Stepanauskas and Edward M. Rubin and P. Bernt Hugenholtz and Tanja Woyke},
  journal={Nature},
  year={2013},
  volume={499},
  pages={431-437}
}
Genome sequencing enhances our understanding of the biological world by providing blueprints for the evolutionary and functional diversity that shapes the biosphere. However, microbial genomes that are currently available are of limited phylogenetic breadth, owing to our historical inability to cultivate most microorganisms in the laboratory. We apply single-cell genomics to target and sequence 201 uncultivated archaeal and bacterial cells from nine diverse habitats belonging to 29 major mostly… 

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...

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