Insights into the mode of action of a putative zinc transporter CzrB in Thermus thermophilus.

@article{Cherezov2008InsightsIT,
  title={Insights into the mode of action of a putative zinc transporter CzrB in Thermus thermophilus.},
  author={Vadim Cherezov and Nicole H{\"o}fer and Doletha M. E. Szebenyi and Olga Kolaj and Joseph G. Wall and Richard E. Gillilan and Vasundara Srinivasan and Christopher P Jaroniec and Martin Caffrey},
  journal={Structure},
  year={2008},
  volume={16 9},
  pages={
          1378-88
        }
}
The crystal structures of the cytoplasmic domain of the putative zinc transporter CzrB in the apo and zinc-bound forms reported herein are consistent with the protein functioning in vivo as a homodimer. NMR, X-ray scattering, and size-exclusion chromatography provide support for dimer formation. Full-length variants of CzrB in the apo and zinc-loaded states were generated by homology modeling with the Zn2+/H+ antiporter YiiP. The model suggests a way in which zinc binding to the cytoplasmic… CONTINUE READING
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