Insights into Pseudomonas putida KT2440 response to phenol‐induced stress by quantitative proteomics

@article{Santos2004InsightsIP,
  title={Insights into Pseudomonas putida KT2440 response to phenol‐induced stress by quantitative proteomics},
  author={Pedro Miguel Santos and Dirk Benndorf and Isabel S{\'a}-Correia},
  journal={PROTEOMICS},
  year={2004},
  volume={4}
}
To gain insight into the global mechanism underlying phenol toxicity and tolerance in bacteria, we have generated a two‐dimensional protein reference map and used it to identify variations in protein expression levels in Pseudomonas putida KT2440 following exposure to sub‐lethal inhibitory concentrations of this solvent. Inspection of the two‐dimensional gel electrophoresis gels revealed that 1 h following sudden cell exposure to two different concentrations of phenol, leading to the inhibition… 
Proteome Analysis of Cellular Response of Pseudomonas putida KT2440 to Tetracycline Stress
TLDR
Results demonstrate that tetracycline at a high concentration induced comprehensive stress in P. putida KT2440 and the global induction of proteins related to bacteria survival and proteome analysis was found to be a useful tool for the elucidation of antibiotic-induced proteins in the present study.
Response of Pseudomonas putida KT2440 to phenol at the level of membrane proteome.
TLDR
Results suggest the important role of protein chaperones, of cell envelope and cell surface and of a more active respiratory chain in the response to phenol, and mechanistic insights may be extended to Pseudomonas adaptation to solvents, of possible impact in biodegradation, bioremediation and biocatalysis.
Mechanistic insights into the global response to phenol in the phenol-biodegrading strain Pseudomonas sp. M1 revealed by quantitative proteomics.
Quantitative proteomics was used to gain insights into the global adaptive response to phenol in the phenol-biodegrading strain Pseudomonas sp. M1 when an alternative carbon source (pyruvate or
A proteome analysis of the yeast response to the herbicide 2,4‐dichlorophenoxyacetic acid
TLDR
Results suggest that during the adaptation period preceding the resumption of inhibited exponential growth under herbicide stress, the antioxidant enzyme Ahp1p and the heat shock proteins Hsp12p and Ssb2p (or Ssb1p) are present in higher amounts.
Physiological comparison of Pseudomonas putida between two growth phases during cometabolism of 4‐chlorophenol in presence of phenol and glutamate: a proteomics approach
TLDR
The expression levels of the identified catabolic enzymes during growth in the two growth phases correlated well with the substrate utilization patterns observed in previous kinetics studies, and the results show that cells growing on a mixture of aromatic substrates undergo significant physiological changes.
Functional Genomics of Stress Response in Pseudomonas putida KT2440
TLDR
Adaptation of the membrane barrier, uptake of phosphate, maintenance of the intracellular pH and redox status, and translational control of metabolism are key mechanisms of the response of P. putida to abiotic stresses.
Protective role of glycerol against benzene stress: insights from the Pseudomonas putida proteome
TLDR
Findings provide evidence to support the hypothesis that hydrophobic substances induce a chaotropicity-mediated water stress, that cells respond via genome-mediated adaptations, and that glycerol protects the cell’s macromolecular systems.
Quantitative analysis of cellular proteome alterations of Pseudomonas putida to naphthalene-induced stress
TLDR
In insights into the cellular response of P. putida to high naphthalene concentrations at the protein level, the expression levels of proteins involved in heat shock and universal stress response and small molecule transport are offered.
Stress responses of Acinetobacter strain Y during phenol degradation
  • Johnson Lin
  • Biology, Medicine
    Archives of Microbiology
  • 2016
TLDR
Bacterial strategies of Acinetobacter strain Y via the energy saving mechanisms and the coordinated control between carbon (C)- and nitrogen (N)-limitations in coping with the stress by scavenging the reactive oxygen species are demonstrated.
Comprehensive proteome analysis of the response of Pseudomonas putida KT2440 to the flavor compound vanillin.
TLDR
The present work will deepen the understanding of metabolism of aromatic compounds in P. putida and may lead to a more comprehensive understanding of solvent tolerance mechanisms in Gram-negative bacteria in general.
...
1
2
3
4
5
...