Insight into the mechanism of the B12-independent glycerol dehydratase from Clostridium butyricum: preliminary biochemical and structural characterization.

@article{OBrien2004InsightIT,
  title={Insight into the mechanism of the B12-independent glycerol dehydratase from Clostridium butyricum: preliminary biochemical and structural characterization.},
  author={Jessica Rae O'Brien and C{\'e}line Raynaud and Christian Croux and Laurence Girbal and Philippe Soucaille and William N Lanzilotta},
  journal={Biochemistry},
  year={2004},
  volume={43 16},
  pages={4635-45}
}
The molecular characterization of a B12-independent glycerol dehydratase from Clostridium butyricum has recently been reported [Raynaud, C., et al. (2003) Proc. Natl. Acad. Sci. U.S.A. 100, 5010-5015]. In this work, we have further characterized this system by biochemical and crystallographic methods. Both the glycerol dehydratase (GD) and the GD-activating enzyme (GD-AE) could be purified to homogeneity under aerobic conditions. In this form, both the GD and GD-AE were inactive. A… CONTINUE READING
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