Inside-out red cell membrane vesicles: preparation and purification.

Abstract

Plasma membranes purified from human red cells were Converted into small vesicleS by disruption in alkaline buffer of low ionic strength. Most of these vesicles were inside-out. The presence of divalent cations prevented this inversion. The inside-out vesicles were separatcd from right-side-out vesicles by centrifugration to equilibrium in dextran density gradients.

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@article{Steck1970InsideoutRC, title={Inside-out red cell membrane vesicles: preparation and purification.}, author={T L Steck and Ronald S. Weinstein and June Hookey Straus and David Wallach}, journal={Science}, year={1970}, volume={168 3928}, pages={255-7} }