Inside-out red cell membrane vesicles: preparation and purification.

  title={Inside-out red cell membrane vesicles: preparation and purification.},
  author={T L Steck and Ronald S. Weinstein and Jose Straus and Donald F. H. Wallach},
  volume={168 3928},
Plasma membranes purified from human red cells were Converted into small vesicleS by disruption in alkaline buffer of low ionic strength. Most of these vesicles were inside-out. The presence of divalent cations prevented this inversion. The inside-out vesicles were separatcd from right-side-out vesicles by centrifugration to equilibrium in dextran density gradients. 

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