Insertional mutagenesis identifies genes that promote the immortalization of primary bone marrow progenitor cells.

@article{Du2005InsertionalMI,
  title={Insertional mutagenesis identifies genes that promote the immortalization of primary bone marrow progenitor cells.},
  author={Yang Du and Nancy A. Jenkins and Neal G. Copeland},
  journal={Blood},
  year={2005},
  volume={106 12},
  pages={3932-9}
}
Retroviruses can induce hematopoietic disease via insertional mutagenesis of cancer genes and provide valuable molecular tags for cancer gene discovery. Here we show that insertional mutagenesis can also identify genes that promote the immortalization of hematopoietic cells, which normally have only limited self-renewal. Transduction of mouse bone marrow cells with replication-incompetent murine stem cell virus (MSCV) expressing only neo, followed by serial passage in liquid culture containing… CONTINUE READING
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