Insertion site and distribution of a genomic island conferring DNA phosphorothioation in the Mycobacterium abscessus complex.

@article{Howard2013InsertionSA,
  title={Insertion site and distribution of a genomic island conferring DNA phosphorothioation in the Mycobacterium abscessus complex.},
  author={Susan T. Howard and Kristopher L Newman and Steven Mcnulty and Barbara A. Brown-Elliott and Ravikiran Vasireddy and Linda Bridge and Richard J. Wallace},
  journal={Microbiology},
  year={2013},
  volume={159 Pt 11},
  pages={2323-32}
}
Nearly half of US clinical isolates of the emerging pathogen Mycobacterium abscessus were reported to exhibit smeared DNA during PFGE. This DNA degradation (Dnd) phenotype results from DNA phosphorothioation, a sulfur modification found in other bacteria and conferred by dnd genes located on mobile elements. Putative dnd genes are located on a 19.6 kbp genomic island (GI) in the M. abscessus type strain ATCC 19977. We confirmed that ATCC 19977(T) is Dnd-positive by PFGE and we developed a PCR… CONTINUE READING