Insertion of specific bases during DNA synthesis past the oxidation-damaged base 8-oxodG

@article{Shibutani1991InsertionOS,
  title={Insertion of specific bases during DNA synthesis past the oxidation-damaged base 8-oxodG},
  author={Shinya Shibutani and Masaru Takeshita and Arthur P. Grollman},
  journal={Nature},
  year={1991},
  volume={349},
  pages={431-434}
}
OXIDATIVE damage to DNA, reflected in the formation of 8-oxo- 7-hydrodeoxyguanosine (8-oxodG)1,2, may be important in mutagenesis, carcinogenesis and the ageing process3,4. Kuchino et al. studied DNA synthesis on oligodeoxynucleotide templates containing 8-oxodG, concluding that the modified base lacked base pairing specificity and directed misreading of pyrimidine residues neighbouring the lesion5. Here we report different results, using an approach in which the several products of a DNA… 

Insertion of dGMP and dAMP during in vitro DNA synthesis opposite an oxidized form of 7,8-dihydro-8-oxoguanine.

TLDR
Results demonstrate that an oxidized form of 8-oxoG, possibly guanidinohydantoin, may direct misreading and misinsertion of dNTPs during DNA synthesis.

Characterization of DNA with an 8-oxoguanine modification

TLDR
Thermodynamic studies show that oxoG destabilizes DNA (ΔΔG of 2–8 kcal mol−1 over a 16–116 mM NaCl range) due to a significant reduction in the enthalpy term, and has a profound effect on the level and nature of DNA hydration indicating that the environment around anOxoG•C is fundamentally different than that found at G•C.

Structure of Human DNA Polymerase κ Inserting dATP Opposite an 8-OxoG DNA Lesion

TLDR
The structure presented here is the first for a eukaryotic translesion synthesis (TLS) DNA polymerase with an 8-oxoG:A base pair in the active site and provides a basis for why Polκ is more efficient at inserting an A opposite the lesion than other Y-family DNA polymerases.

Base excision repair synthesis of DNA containing 8-oxoguanine in Escherichia coli

TLDR
The results indicate the biological significance of the glycosylase and apurinic/ apyrimidinic lyase activities of Fpg, in concert with 3' phosphatase(s) to create an appropriately gapped substrate for efficient BER synthesis of DNA containing 8-oxo-G.

DNA polymerase minor groove interactions modulate mutagenic bypass of a templating 8-oxoguanine lesion

TLDR
The results demonstrate that the incoming nucleotide is unable to induce a syn-8-oxoG conformation without minor groove DNA polymerase interactions that influence templating (anti-/syn-equilibrium) of 8-OxoG while modulating fidelity.

Action of Mitochondrial DNA Polymerase at Sites of Base Loss or Oxidative Damage (*)

TLDR
Both mitochondrial DNA lesions have the prospect of causing high rates of mutation during mtDNA replication and the effects of these lesions on the 3′ 5′ exonuclease proofreading activity of DNA polymerase were investigated.

Recognition and Repair of 8‐Oxoguanine and Formamidopyrimidine Lesions in DNA a

TLDR
Plasmid vectors containing a single lesion were used to establish the mutational frequency and specificity of 9oxodG in bacteria and mammalian cells; 9-OxodG is weakly mutagenic in both systems; G:C{l_arrow}T:A transversions are targeted to the site of the lesion.
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