Insertion of a reamplification round into the ISSR-PCR protocol gives new flax fingerprinting patterns.

Abstract

We expanded the basic ISSR-PCR protocol by an additional PCR reamplification round in order to detect whether increased PCR productivity would give new bands in ISSR patterns. We found that the reamplification step had a prominent impact on the quality of the inter-simple-sequence repeat (ISSR) PCR patterns of flax, depending on the particular primer used… (More)

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