Insertion of DNA sequences into the human chromosomal β-globin locus by homologous recombination

@article{Smithies1985InsertionOD,
  title={Insertion of DNA sequences into the human chromosomal $\beta$-globin locus by homologous recombination},
  author={Oliver Smithies and Ronald G. Gregg and Sallie S. Boggs and Michał Koralewski and Raju Kucherlapati},
  journal={Nature},
  year={1985},
  volume={317},
  pages={230-234}
}
A ‘rescuable’ plasmid containing globin gene sequences allowing recombination with homologous chromosomal sequences has enabled us to produce, score and clone mammalian cells with the plasmid integrated into the human β-globin locus. The planned modification was achieved in about one per thousand transformed cells whether or not the target gene was expressed. 

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...

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