Insect transformation with piggyBac: getting the number of injections just right

@inproceedings{Gregory2016InsectTW,
  title={Insect transformation with piggyBac: getting the number of injections just right},
  author={Maggie Gregory and Luke S. Alphey and Neil I. Morrison and Sebastian M Shimeld},
  booktitle={Insect molecular biology},
  year={2016}
}
The insertion of exogenous genetic cargo into insects using transposable elements is a powerful research tool with potential applications in meeting food security and public health challenges facing humanity. piggyBac is the transposable element most commonly utilized for insect germline transformation. The described efficiency of this process is variable in the published literature, and a comprehensive review of transformation efficiency in insects is lacking. This study compared and… CONTINUE READING

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