Insect-specific flaviviruses from Culex mosquitoes in Colorado, with evidence of vertical transmission.

@article{Bolling2011InsectspecificFF,
  title={Insect-specific flaviviruses from Culex mosquitoes in Colorado, with evidence of vertical transmission.},
  author={Bethany G. Bolling and Lars Eisen and Chester G. Moore and Carol D. Blair},
  journal={The American journal of tropical medicine and hygiene},
  year={2011},
  volume={85 1},
  pages={
          169-77
        }
}
Mosquitoes were collected in Colorado during 2006 and 2007 to examine spatial and seasonal patterns of risk for exposure to Culex vectors and West Nile virus. We used universal flavivirus primers to test pools of Culex mosquitoes for viral RNA. This led to the detection and subsequent isolation of two insect-specific flaviviruses: Culex flavivirus (CxFV), which was first described from Japan, and a novel insect flavivirus, designated Calbertado virus (CLBOV), which has also been detected in… 

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