Insect larval expression process is optimized by generating fusions with green fluorescent protein.

@article{Cha1999InsectLE,
  title={Insect larval expression process is optimized by generating fusions with green fluorescent protein.},
  author={Hyung Joon Cha and Nimish G. Dalal and Michelle Q Pham and Vikram N. Vakharia and Govind Rao and William E. Bentley},
  journal={Biotechnology and bioengineering},
  year={1999},
  volume={65 3},
  pages={316-24}
}
The insect larvae/baculovirus protein production process was dramatically simplified by expressing fusion proteins containing green fluorescent protein (GFP) and the product-of-interest. In this case, human interleukin-2 (hIL-2) and chloramphenicol acetyl-transferase (CAT) were model products. Specifically, our fusion construct was comprised of a histidine affinity ligand for simplified purification using immobilized metal affinity chromatography (IMAC), the UV-optimized GFP (GFPuv) as a marker… CONTINUE READING

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