Insect cells contain an unusual, membrane-bound beta-N-acetylglucosaminidase probably involved in the processing of protein N-glycans.

@article{Altmann1995InsectCC,
  title={Insect cells contain an unusual, membrane-bound beta-N-acetylglucosaminidase probably involved in the processing of protein N-glycans.},
  author={Friedrich Altmann and Herwig Schwihla and Erika Staudacher and Josef Gl{\"o}ssl and Leopold M{\"a}rz},
  journal={The Journal of biological chemistry},
  year={1995},
  volume={270 29},
  pages={17344-9}
}
The beta-N-acetylglucosaminidase activity in the lepidopteran insect cell line Sf21 has been studied using pyridylaminated oligosaccharides and chromogenic synthetic glycosides as substrates. Ultracentrifugation experiments indicated that the insect cell beta-N-acetylglucosminidase exists in a soluble and a membrane-bound form. This latter form accounted for two-thirds of the total activity and was associated with vesicles of the same density as those containing GlcNAc-transferase I. Partial… CONTINUE READING

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