Insect cells as hosts for the expression of recombinant glycoproteins

  title={Insect cells as hosts for the expression of recombinant glycoproteins},
  author={Friedrich Altmann and Erika Staudacher and Iain B. H. Wilson and Leopold M{\"a}rz},
  journal={Glycoconjugate Journal},
Baculovirus-mediated expression in insect cells has become well-established for the production of recombinant glycoproteins. Its frequent use arises from the relative ease and speed with which a heterologous protein can be expressed on the laboratory scale and the high chance of obtaining a biologically active protein. In addition to Spodoptera frugiperda Sf9 cells, which are probably the most widely used insect cell line, other mainly lepidopteran cell lines are exploited for protein… 
The stable transfection of insect cells is an alternative to BEVS which has recently been augmented with recombinase-mediated cassette exchange for site-specific gene integration.
Baculovirus Vectors
  • A. Hüser, C. Hofmann
  • Biology, Engineering
    American journal of pharmacogenomics : genomics-related research in drug development and clinical practice
  • 2003
Baculovirus vectors are able to deliver very large DNA sequences into mammalian cells and vectors for toxic gene products can also be generated, and are valuable tools for launching viral infection in cases where there is no appropriate cell culture system.
Production of therapeutic proteins with baculovirus expression system in insect cell
This review presents a brief overview of the perspective, particularly the glycosylation aspect, of the production of therapeutic recombinant proteins via a baculovirus‐based insect cell expression system.
Transient Expression and Cellular Localization of Recombinant Proteins in Cultured Insect Cells.
A method for generating nonlytic expression vectors and transient recombinant protein expression that allows for the efficient cellular localization of recombinant proteins and can be used to rapidly discern protein trafficking within the cell is described.
SweetBac: A New Approach for the Production of Mammalianised Glycoproteins in Insect Cells
The overall expression rate, especially in the recently established Tnao38 cell line, was comparable to that of transient expression in mammalian cells, and the ability of SweetBac to generate mammalian-like N-glycan structures on 3D6 antibody was evaluated.
Expression and Production of Human Interleukin-7 in Insect Cells Using Baculovirus Expression Vector System (BEVS)
The production of recombinant human interleukin-7 (hIL-7) in insect cells shows the efficacy of this system for large-scale production and offers a highly efficient, inexpensive, and convenient system.
Process Optimization for Recombinant Protein Expression in Insect Cells
This chapter describes the setup of both baculovirus expression vector system in Spodoptera frugiperda cells and the stable transformation of Drosophila melanogaster S2 cells, including steps for the generation of expression vectors and comprehensive optimization approaches.
Protein N-glycosylation in the baculovirus-insect cell system.
This chapter discusses the models of insect protein N-glycosylation that have emerged from a wide variety of studies and how this impacts the use of baculovirus-insect cell systems for recombinant glycoprotein production.
Transduction of HEK293 Cells with BacMam Baculovirus Is an Efficient System for the Production of HIV-1 Virus-like Particles
This work puts the basis for future studies aiming at scaling up the BacMam baculovirus system as an alternative strategy for VLP production by determining the optimal conditions of multiplicity of infection (MOI), viable cell density (VCD) at infection, and butyric acid (BA) concentration that maximize cell transduction and V LP production.