Inotropic Response of Cardiac Ventricular Myocytes to &bgr;-Adrenergic Stimulation With Isoproterenol Exhibits Diurnal Variation: Involvement of Nitric Oxide

@article{Collins2010InotropicRO,
  title={Inotropic Response of Cardiac Ventricular Myocytes to \&bgr;-Adrenergic Stimulation With Isoproterenol Exhibits Diurnal Variation: Involvement of Nitric Oxide},
  author={Helen E Collins and Glenn C. Rodrigo},
  journal={Circulation Research},
  year={2010},
  volume={106},
  pages={1244-1252}
}
Rationale: Although >10% of cardiac gene expression displays diurnal variations, little is known of their impact on excitation–contraction coupling. Objective: To determine whether the time of day affects excitation–contraction coupling in rat ventricles. Methods and Results: Left ventricular myocytes were isolated from rat hearts at 2 opposing time points, corresponding to the animals resting or active periods. Basal contraction and [Ca2+]i was significantly greater in myocytes isolated during… 

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