Inorganic Pyrophosphatase Activity of Purified Bovine Pulp Alkaline Phosphatase at Physiological pH

@article{Harada1986InorganicPA,
  title={Inorganic Pyrophosphatase Activity of Purified Bovine Pulp Alkaline Phosphatase at Physiological pH},
  author={M. Harada and N. Udagawa and K. Fukasawa and B. Y. Hiraoka and M. Mogi},
  journal={Journal of Dental Research},
  year={1986},
  volume={65},
  pages={125 - 127}
}
  • M. Harada, N. Udagawa, +2 authors M. Mogi
  • Published 1986
  • Medicine
  • Journal of Dental Research
  • At physiological pH, the hydrolytic activity of purified bovine pulp alkaline phosphatase toward phosphorus compounds was observed to be in the order of inorganic pyrophosphate > β-glycerophosphate > phosphorylcholine > p-nitrophenylphosphate > glucose-6-phosphate. Optimum pH of the enzyme toward inorganic pyrophosphate was shown to be 8.5, with around 60% of the activity at pH 7.5. The activity was increased by the addition of Mg2+, but a different pattern of activation was observed between pH… CONTINUE READING
    42 Citations
    Kinetic Studies of Alkaline Phosphatase from the Liver of Agama agama Lizard
    • 2
    • Highly Influenced
    • PDF
    Hypophosphatasia and the extracellular metabolism of inorganic pyrophosphate: clinical and laboratory aspects.
    • 95

    References

    SHOWING 1-10 OF 17 REFERENCES
    The pyrophosphatase and (Ca2+-Mg2+)-ATPase activity of purified calf bone alkaline phosphatase.
    • 53
    Purification and properties of bovine dental-pulp alkaline-phosphatase.
    • 13
    Alkaline phosphatase.
    • 487
    • PDF
    Mechanism of Calcification: Inhibitory Role of Pyrophosphate
    • 203
    Separation of human tissue alkaline phosphatases by electrophoresis on acrylamide disc gels.
    • 158