Inoculum Size of Clostridium botulinum 56 A Spores Influences Time-to-Detection and Percent Growth-Positive Samples

@inproceedings{ZhaoInoculumSO,
  title={Inoculum Size of Clostridium botulinum 56 A Spores Influences Time-to-Detection and Percent Growth-Positive Samples},
  author={Liqiang Zhao and Thomas J. Montville and Donald W Schaffner}
}
The influence of inoculum size on the growth kinetics of Clostridium botulinum 56A and percentage of growth-positive samples was studied in a complete factorial design with factors of inoculum size (1, 100, or 10,000 spores), pH, and sodium-chloride concentration. Growth was followed hourly as change in A 620. Polynomial regression was used to analyze the data. The time-to-detection and percent growth-positive samples were significantly affected by inoculum size and its quadratic term. When… CONTINUE READING

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